Comparative study of sperm washing and selection methods after cryopreservation and its influence on sperm subpopulational structure in a bovine model.
- Authors
- Garc?a Herreros, Manuel
- Format
- Article
- Status
- publishedVersion
- Description
The effect of different sperm washing-selection methods on sperm morphometric characteristics as a study to detect differences in the subpopulational structure has been carried out in detail in a bovine model. Cryopreserved sperm samples from 5 bulls were thawed, pooled, and processed by TALP-washing centrifugation method (TWCM), selective Percoll discontinuous density-gradient centrifugation method (PDGM), and self-migration swim-up separation method (SUMM). Live-dead assay (SYBR-14/ethidium homodimer-1), chlortetracycline assay (CTC), and sperm motility were assessed, and aliquots of sperm were processed for automated sperm morphometry analysis (ASMA) simultaneously before (raw thawed sperm used as control, RTS) and after different sperm washing-selection techniques. Deleterious effects of different methods were evident, particularly on sperm membrane integrity (p?Universidad T?cnica Del Cotopaxi
http://www.ncbi.nlm.nih.gov/pubmed/25007138
- Publication Year
- 2014
- Language
- eng
- Topic
- BOVINE MODEL
PRINCIPAL COMPONENT ANALYSIS
SPERM SELECTION
SPERM SUBPOPULATIONAL
- Repository
- Repositorio SENESCYT
- Rights
- openAccess
- License
- openAccess