Molecular diagnostics to identify fungal plant pathogens ? A review of current methods
- Authors
- Bernreiter, Andreas
- Format
- Article
- Status
- publishedVersion
- Description
La siguiente revisi?n ofrece una visi?n amplia de los m?todos actuales utilizados para la identificaci?n de hongos fitopat?genos. Adem?s, se presentan estudios previos centrados en pat?genos relevantes para el Ecuador y una breve discusi?n sobre el futuro del diagn?stico en fitopatolog?a. En la actualidad, las herramientas de diagn?stico que aplican biolog?a molecular se basan en la tecnolog?a de reacci?n en cadena de la polimerasa (PCR). Se presenta una selecci?n de tecnolog?as basadas en PCR y se discuten sus ventajas y desventajas. Igualmente, las tecnolog?as de punta utilizadas para sequenciacion (NGS), son una poderosa herramienta de diagn?stico, que presentan la ventaja de permitir la identificaci?n simult?nea de m?ltiples infecciones y es capaz de descubrir agentes pat?genos desconocidos. Los m?todos basados en espectrometr?a de masas son utilizados principalmente para diagn?stico cl?nico, pero mediante un acertado desarrollo de protocolos, este m?todo tambi?n se aplicara a patolog?a vegetal. Las enfermedades de las plantas a menudo son causadas por hongos y con efectos generalmente m?s severos que los causados por virus o bacterias. Hasta ahora la identificaci?n de hongos en laboratorios se realiz? principalmente mediante m?todos convencionales, tales como morfolog?a de las colonias y sus caracter?sticas microsc?picas, pero tales estudios morfol?gicos consumen tiempo y pueden conducir a resultados err?neos.
The following review gives a broad overview of current methods for identification of fungal plant pathogens. Furthermore, some selected previous studies with focus on relevant pathogens for Ecuador are presented and there will be a brief discussion to the future of molecular diagnostics in plant pathology. Currently, most molecular diagnostic tools are based on the polymerase chain reaction (PCR) technology. A selection of technologies based on PCR is presented and the advantages and disadvantages are discussed. Next generation sequencing (NGS) is a powerful new diagnostic tool, which has the advantage for simultaneous identification of multiple infections and is able to discover new and unknown pathogens. Mass spectrometry methods are so far mostly tested in clinical diagnostics, but upon successful establishment of diagnostic procedures, applications in plant pathology will follow in near future. Plant diseases are often caused by fungal or fungal-like organisms and the effect on the host plants is usually more severe than effects caused by viruses or bacteria. Identification of fungi in laboratories was until now primarily performed by conventional methods such as colony morphology and microscopic characteristics, but such morphological studies are time consuming and can lead to erroneous outcomes.
http://www.agrocalidad.gob.ec/revistaecuadorescalidad/index.php/revista/article/view/59/106
- Publication Year
- 2017
- Language
- eng
- Topic
- PCR
NEXT-GENERATION SEQUENCING
SANGER SEQUENCING
MOLECULAR DIAGNOSTICS
FUSARIUM
- Repository
- Repositorio SENESCYT
- Rights
- openAccess
- License
- openAccess