Aislamiento, cultivo e identificación molecular de células madre espermatogoniales en bovinos
- Authors
- Erazo Romero, Saulo
- Format
- BachelorThesis
- Status
- publishedVersion
- Description
Bovine spermatogonial stem cells (SSC) were isolated, cultured and molecularly identified. Three trials were made from the testes of three calves between one and a half months and four months of age. For cell isolation, two enzymatic digestions were performed and type A spermatogonia were purified by differential plating. During culture, three concentrations of fetal calf serum (FCS), 0.25%, 1% and 10% were tested...
En este trabajo se aislaron, cultivaron e identificaron de forma molecular células madre espermatogoniales (SSC) en bovinos. Se realizaron tres ensayos a partir de los testículos de tres terneros de entre un mes y medio y cuatro meses de edad. Para el aislamiento se realizaron dos digestiones enzimáticas y se purificaron las espermatogonias tipo A mediante la técnica de plaqueo diferencial...
- Publication Year
- 2017
- Language
- spa
- Topic
- Células madre -- Animales -- Biotecnología -- Tesis y disertaciones académicas.
Cultivo de células -- Uso terapéutico.
Tecnología
Tecnología química
- Repository
- Repositorio Universidad San Francisco de Quito
- Get full text
- http://repositorio.usfq.edu.ec/handle/23000/6840
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/3.0/ec/